KRAS Knockout cell line(RKO)

KRAS Knockout cell line(RKO) Order

Catalog#

YKO-H251

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

KRAS Knockout cell line (RKO)

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

KRAS

Gene ID

3845

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Target region

Knockout region: Exon 2 (based on transcript KRAS-202)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:RKO			STR Info (Cell bank) Cell Line:RKO
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	13		11	13
D13S317	8	11		8	11
D7S820	8	10		8	10
D16S539	13	13		12	13
VWA	16	22		16	22
TH01	6	10		6	10
AMEL	X	X		X	X
TPOX	11	11		11	11
CSF1PO	8	10		8	10

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the KRAS gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RKO cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, KRAS gene knockout KRAS Knockout cell line (RKO) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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